ABSTRACT
To evaluate the status of the testes, thyroid, and adrenals in male alcoholics during the period of voluntary abstinence and therapy, chronic male drinkers undergoing a 4 wk inpatient deaddiction programme in a social hospital were recruited. Levels of a few serum and urinary hormones/metabolites viz., serum testosterone, total triiodothyronine (T3) and thyroxine (T4) and urinary total 17-ketosteroids (17-KS), estrone, estradiol, and 17-hydroxy corticosteroids (17-OHCS) were assessed in alcoholics thrice during the treatment programme at hospital i.e., on the zero (day of admission), 10th, and 20th day and compared to those of non-alcoholic controls. Alcoholics registered elevated serum total T3, and reduced total T4 and testosterone levels at admission, which persisted even after 20 days of the rehabilitative programme. Markedly high urinary levels of total 17-KS, estrone, and 17-OHCS were observed on zero day of admission. Urinary estrone and 17-OHCS, unlike total 17-KS, showed a trend to return to the normal range during the 20 days period. Urinary estradiol levels, however, recorded no significant alteration. The results of this preliminary study are suggestive of alcohol-induced perturbations on the functional integrity of the testes, thyroid, and adrenal in male alcohol addicts, wherein 20 days period of total alcohol abstinence and rehabilitative programme failed to reverse alcohol-induced hypoandrogenization and altered thyroidal status, but only partially restored certain biochemical events associated with the excretion of steroid metabolites.
Subject(s)
17-Hydroxycorticosteroids/urine , 17-Ketosteroids/urine , Adrenal Cortex/physiopathology , Adult , Alcoholism/physiopathology , Estradiol/urine , Estrone/urine , Humans , Male , Testis/physiopathology , Testosterone/blood , Thyroid Gland/physiopathology , Thyroxine/blood , Triiodothyronine/bloodABSTRACT
En la anamnesis de la hirsuta, destaca el inicio peripuberal del hirsutismo y en el examen físico la importancia del vello pubiano que aporta el 40 por ciento del score total. Del 74 por ciento de hirsutas hiperandrogénicas, el 34 por ciento poseen elevación exclusiva de la testosterona, el 24 por ciento de la dehidroepiandrosterona sulfato y el 42 por ciento un alza de ambas. El alto porcentaje de dehidroepiandrosterona sulfato aumentada, sola o junto a testosterona, sugiere un compromiso suprarrenal en la etiología del hirsutismo, respaldado por un 50 por ciento de prueba de estimulación suprarrenal con hiperrespuesta de este andrógeno, en hirsutas y en pacientes con síndrome de ovario poliquístico. El alza de la 17 hidroxiprogesterona basal, obliga a realizar prueba de estimulación cob ACTH para descartar su origen ov+arico y confirmar el déficit de la 21 hidroxilasa. Un 5 por ciento de las hirsutas tenían acantosis nigricans con resistencia insulínica y alteraciones lipídicas. Se analiza el tratamiento con antiandrógenos, ciproterona y espironolactona y la frenación con glucocorticoides
Subject(s)
Humans , Female , Adolescent , Adult , Endocrine System Diseases/complications , Hirsutism/etiology , 17-Ketosteroids/urine , Acanthosis Nigricans/diagnosis , Cyproterone/administration & dosage , Ethinyl Estradiol/administration & dosage , Physical Examination/methods , Hirsutism/diagnosis , Hirsutism/drug therapy , Hormones/metabolism , Hyperandrogenism/diagnosis , Insulin Resistance , Testosterone/metabolismABSTRACT
Twenty eight histologically confirmed cases of Hodgkin's disease were evaluates with regard to libido, sperm count, FSH, LH and urinary ketosteroid levels, before and during different stages of chemotherapy along with testicular biopsy on 16 cases. Decrease in libido during therapy improved following treatment, 50% cases who were oligospermic before treatment became azospermic, Serum FSH levels increased significantly (p 0.001) during the course of treatment. There was no significant change in LH and urinary ketosteroid levels (p 0.05). Testicular biopsy, normal initially, showed germ cell aplasia and absence of spermatogenesis after therapy. Drug induced testicular change leads to sterility.
Subject(s)
17-Ketosteroids/urine , Adolescent , Adult , Combined Modality Therapy , Follicle Stimulating Hormone/blood , Hodgkin Disease/metabolism , Humans , Libido , Luteinizing Hormone/blood , Male , Middle Aged , Sperm CountABSTRACT
The effects of chronic administration of kat [Calha edulis] on several physiological and biochemical parameters of the male rats [Rattus norvegicus] were studied. Cholesterol and ascorbic acid contents of the adrenals were found to be gradually declined as a result of kat-administration. Neurotransmitters in the brain appeared to be affected by kat-administration. Norepinephrine content was significantly decreased and acetylcholine significantly increased in kat-treated than in control rats. However, no significant changes in serotonin level were found. Kat administration also affected the plasma testosterone level; an initial rise occurred during the first four months probably as a result of the stimulating effect of kat, then a general decrease was observed until the termination of the experiment. On the other hand, kat-treated rats appeared to have no significant changes in the urinary 17-ketosteroid level throughout the experiment
Subject(s)
Animals, Laboratory , Brain , Cholesterol , Ascorbic Acid/blood , Catecholamines , Testosterone , 17-Ketosteroids/urine , RatsABSTRACT
Estimation of plasma cortisol by fluorometric technique desorbed by Mallingly's was carried out in 56 cases, including 38 cases of myopia (19 cases of simple myopia and 19 cases of degenerative myopia) and 18 normal individuals. Urinary 17-keto steroids/24 hours was also estimated by Zimmermann technique in 12 out of the 56 cases, which include 8 cases of myopia (4 cases of simple myopia and 4 cases of degenerative myopia) and 4 normal individuals as control. Plasma cortisol level is lower in simple and degenerative myopia than in normal subjects, but on statistical analysis the difference was not of much significance. Patients with simple myopia with positive family history showed lower value of plasma cortisol than patients with simple myopia with negative family history, the difference was also statistically insignificant (P-0.1). In degenerative myopia patients, with and without family history, there was very little difference in plasma cortisol level and statistically highly insignificant (P-0.8). Urinary 17 keto steroid/24 hour values are lower in simple and degenerative myopia than in normal subjects.
Subject(s)
17-Ketosteroids/urine , Adolescent , Adult , Female , Humans , Hydrocortisone/blood , Male , Myopia/bloodABSTRACT
A atividade endócrina relacionada com os órgäos sexuais foi avaliada em mulheres menopausadas e pós-menopausadas, antes e depois da ressecçäo cirúrgica bilateral dos ovários. Comparando os valores urinários das gonadotrofinas dos 17-cetoesteróides e estrogênios, comprova-se uma persistente atividade endócrina nos ovários senis
Subject(s)
Humans , Female , Middle Aged , 17-Ketosteroids/urine , Estrogens/urine , Genitalia/metabolism , Gonadotropins/urine , Ovariectomy , Postmenopause/metabolismABSTRACT
Se desarrolló una microtécnica para la cuantificación de 17-cetoesteroides en orina de 24 horas. Se procesaron 75 muestras de orina con concentraciones bajas, medias y altas. Al analizar las muestras por la microtécnica se obtiene un ahorro de 66% en reactivos y por ende, en costo por prueba. Se encontró excelente correlación entre el micrométodo y macrotécnica, p <0.001, r = 0.99. Por lo tanto, esta técnica es recomendable por su excelente reproducibilidad y economía
Subject(s)
Humans , 17-Ketosteroids/urine , Electron Probe Microanalysis/methodsABSTRACT
Se presenta un método de fraccionamiento de 17-Cetoesteroides (17-Ce) neutros urinarios, mediante dos cromatografías en capa delgada sucesivas: la primera, sobre sílica gel, separa en forma adecuada androstenodiona (delta 4) etiocoloanoloma (E) y derivados 11 oxigenados (C19 O3); la segunda, sobre óxido de aluminio y doble desarrollo, separa convenientemente dehidroepiandrosterona (DHEA) de adrosterona (A). Para ambas se utiliza el sistema de solventes compuesto por cloruro de metileno: etanol (97:3). Las determinaciones cuantitativas se realizan aplicaciones intra e interensayos por triplicado fueron: para C19 O3 ñ 3,62 y ñ 4,31; E, ñ 4,94 y ñ delta 4, ñ 2,08 y ñ 6,27; DHEA, ñ 6,66 y ñ 7,45; A, ñ 3,00 y ñ 13,26, respectivamente. La exactitud del método oscila entre 92,1 y 106,8%. En un cuadro comparativo se ordenan, además, los valores de referencia del método y los referidos por algunos autores en nuestro páis y en el extranjero. El método es relativamente sencillo y reproducible; requiere de instrumental accesible y logra un fraccionamiento satisfactorio de los 17-Ce más importantes en el manejo del laboratorio clínico
Subject(s)
Humans , 17-Ketosteroids/urine , Chemical Fractionation , Chromatography, Thin Layer , In Vitro TechniquesABSTRACT
Uma paciente com síndrome de Sheehan (necrose pituitária pós-parto) desenvolveu gravidez a termo, quatro anos após a instalaçäo do hipopituitarismo, na vigência de reposiçäp hormonal tiroidiana e corticosteróide. Testes de estímulo da pituitária demonstraram claramente que o setor gonadotrófico era responsivo, sugerindo que possivelmente as células gonadotróficas teriam sido poupadas, pelo menos parcialmente, o suficiente para que houvesse ovulaçäo espontânea.